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Document Details
Document Type
:
Article In Journal
Document Title
:
Comparative Transcriptomics between Synechococcus PCC 7942 and Synechocystis PCC 6803 Provide Insights into Mechanisms of Stress Acclimation
Comparative Transcriptomics between Synechococcus PCC 7942 and Synechocystis PCC 6803 Provide Insights into Mechanisms of Stress Acclimation
Document Language
:
English
Abstract
:
Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803 are model cyanobacteria from which the metabolism and adaptive responses of other cyanobacteria are inferred. Using stranded and 5' enriched libraries, we measured the gene expression response of cells transferred from reference conditions to stress conditions of decreased inorganic carbon, increased salinity, increased pH, and decreased illumination at 1-h and 24-h after transfer. We found that the specific responses of the two strains were by no means identical. Transcriptome profiles allowed us to improve the structural annotation of the genome i.e. identify possible missed genes (including anti-sense), alter gene coordinates and determine transcriptional units (operons). Finally, we predicted associations between proteins of unknown function and biochemical pathways by revealing proteins of known functions that are co-regulated with the unknowns. Future studies of these model organisms will benefit from the cataloging of their responses to environmentally relevant stresses, and improvements in their genome annotations found here.
ISSN
:
1932-6203
Journal Name
:
PLOS ONE
Volume
:
9
Issue Number
:
10
Publishing Year
:
1435 AH
2014 AD
Article Type
:
Article
Added Date
:
Monday, August 14, 2017
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
Konstantinos Billis
Billis, Konstantinos
Researcher
Maria Billini
Billini, Maria
Researcher
Nikos Kyrpides
Kyrpides, Nikos
Researcher
Konstantinos Mavromatis
Mavromatis, Konstantinos
Researcher
H Tripp
Tripp, h
Researcher
Files
File Name
Type
Description
42623.pdf
pdf
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