Document Details

Document Type : Article In Journal 
Document Title :
Genomic and transcriptomic analysis of Laccaria bicolor CAZome reveals insights into polysaccharides remodelling during symbiosis establishment
Genomic and transcriptomic analysis of Laccaria bicolor CAZome reveals insights into polysaccharides remodelling during symbiosis establishment
 
Document Language : English 
Abstract : Ectomycorrhizal fungi, living in soil forests, are required microorganisms to sustain tree growth and productivity. The establishment of mutualistic interaction with roots to form ectomycorrhiza (ECM) is not well known at the molecular level. In particular, how fungal and plant cell walls are rearranged to establish a fully functional ectomycorrhiza is poorly understood. Nevertheless, it is likely that Carbohydrate Active enZymes (CAZyme) produced by the fungus participate in this process. Genome-wide transcriptome profiling during ECM development was used to examine how the CAZome of Laccaria bicolor is regulated during symbiosis establishment. CAZymes active on fungal cell wall were upregulated during ECM development in particular after 4 weeks of contact when the hyphae are surrounding the root cells and start to colonize the apoplast. We demonstrated that one expansin-like protein, whose expression is specific to symbiotic tissues, localizes within fungal cell wall. Whereas L. bicolor genome contained a constricted repertoire of CAZymes active on cellulose and hemicellulose, these CAZymes were expressed during the first steps of root cells colonization. L bicolor retained the ability to use homogalacturonan, a pectin-derived substrate, as carbon source. CAZymes likely involved in pectin hydrolysis were mainly expressed at the stage of a fully mature ECM. All together, our data suggest an active remodelling of fungal cell wall with a possible involvement of expansin during ECM development. By contrast, a soft remodelling of the plant cell wall likely occurs through the loosening of the cellulose microfibrils by AA9 or GH12 CAZymes and middle lamella smooth remodelling through pectin (homogalacturonan) hydrolysis likely by GH28, GH12 CAZymes. (C) 2014 Elsevier Inc. All rights reserved. 
ISSN : 1087-1845 
Journal Name : FUNGAL GENETICS AND BIOLOGY 
Volume : 72 
Issue Number : 1 
Publishing Year : 1435 AH
2014 AD 
Article Type : Article 
Added Date : Wednesday, August 2, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Claire Veneault-FourreyVeneault-Fourrey, Claire Researcher  
Carine CommunCommun, Carine Researcher  
Annegret KohlerKohler, Annegret Researcher  
Emmanuelle MorinMorin, Emmanuelle Researcher  
Raffaella BalestriniBalestrini, Raffaella Researcher  
Jonathan PlettPlett, Jonathan Researcher  
Etienne DanchinDanchin, Etienne Researcher  
Pedro CoutinhoCoutinho, Pedro Researcher  
Ad WiebengaWiebenga, Ad Researcher  
Ronald de Vriesde Vries, Ronald Researcher  
Bernard HenrissatHenrissat, Bernard Researcher  
Francisco MartinMartin, Francis Researcher  

Files

File NameTypeDescription
 42417.pdf pdf 

Back To Researches Page