Document Details

Document Type : Article In Journal 
Document Title :
Genotoxicity of waterpipe smoke in buccal cells and peripheral blood leukocytes as determined by comet assay
Genotoxicity of waterpipe smoke in buccal cells and peripheral blood leukocytes as determined by comet assay
 
Document Language : English 
Abstract : Context: Waterpipe smoke causes DNA damage in peripheral blood leukocytes and in buccal cells of smokers. Objective: To determine the exposure effect of waterpipe smoke on buccal cells and peripheral blood leukocytes in regard to DNA damage using comet assay. Materials and methods: The waterpipe smoke condensates were analyzed by gas chromatography-mass spectrometry (GC-MS). The study was performed on 20 waterpipe smokers. To perform comet assay on bucaal cells of smokers, 10 mu l of cell suspension was mixed with 85 mu l of pre-warmed 1% low melting agarose, applied to comet slide and electrophoresed. To analyze the effect of smoke condensate in vitro, 1 ml of peripheral blood was mixed with 10 mu l of smoke condensate and subjected for comet assay. Results: The GC-MS analysis revealed the presence of 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4on, nicotine, hydroxymethyl furancarboxaldehyde and 3-ethoxy-4-hydroxybenzaldehyde in the smoke condensates. Waterpipe smoking caused DNA damage in vivo in buccal cells of smokers. The tail moment and tail length in buccal cells of smokers were 186 +/- 26 and 456 +/- 71, respectively, which are higher than control. The jurak and moassel smoke condensates were found to cause DNA damage in peripheral blood leukocytes. The moassel smoke condensate was more damaging. Discussion: There is wide misconception that waterpipe smoking is not as harmful as cigarette smoking. This study demonstrated that waterpipe smoke induced DNA damage in exposed cells. Conclusion: Waterpipe smokes cause DNA damage in buccal cells. The smoke condensate of both jurak and moassel caused comet formation suggesting DNA damage in peripheral blood leukocytes. 
ISSN : 0895-8378 
Journal Name : INHALATION TOXICOLOGY 
Volume : 26 
Issue Number : 14 
Publishing Year : 1435 AH
2014 AD 
Article Type : Article 
Added Date : Tuesday, August 1, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Hadba (Al-Amrah(Al-Amrah, Hadba Researcher  
Osama AboznadaAboznada, Osama Researcher  
Alam AlamAlam, Alam Researcher  
M-Zaki ElAssouliElAssouli, M-Zaki Researcher  
Mohammad MujallidMujallid, Mohammad Researcher  
Sufian ElAssouliElAssouli, Sufian Researcher  

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