Document Details
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Document Type |
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Article In Journal |
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Document Title |
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Characterization and DNA-Binding Specificities of Ralstonia TAL-Like Effectors Characterization and DNA-Binding Specificities of Ralstonia TAL-Like Effectors |
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Document Language |
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English |
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Abstract |
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We report the characterization of three Ralstonia TAL-like effectors, which mediate DNA binding and can be used as customizable architectures for DNA targeting. We determined DNA-binding specificities of novel repeat variable di-residues (RVDs) and devised a repeat assembly approach for engineering Ralstonia solanacearum TALE-like proteins (RTLs).Transcription activator-like effectors (TALEs) from Xanthomonas sp. have been used as customizable DNA-binding modules for genome-engineering applications. Ralstonia solanacearum TALE-like proteins (RTLs) exhibit similar structural features to TALEs, including a central DNA-binding domain composed of 35 amino acid-long repeats. Here, we characterize the RTLs and show that they localize in the plant cell nucleus, mediate DNA binding, and might function as transcriptional activators. RTLs have a unique DNA-binding architecture and are enriched in repeat variable di-residues (RVDs), which determine repeat DNA-binding specificities. We determined the DNA-binding specificities for the RVD sequences ND, HN, NP, and NT. The RVD ND mediates highly specific interactions with C nucleotide, HN interacts specifically with A and G nucleotides, and NP binds to C, A, and G nucleotides. Moreover, we developed a highly efficient repeat assembly approach for engineering RTL effectors. Taken together, our data demonstrate that RTLs are unique DNA-targeting modules that are excellent alternatives to be tailored to bind to user-selected DNA sequences for targeted genomic and epigenomic modifications. These findings will facilitate research concerning RTL molecular biology and RTL roles in the pathogenicity of Ralstonia spp. |
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ISSN |
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1674-2052 |
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Journal Name |
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MOLECULAR PLANT |
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Volume |
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6 |
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Issue Number |
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4 |
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Publishing Year |
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1434 AH
2013 AD |
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Article Type |
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Article |
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Added Date |
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Tuesday, July 25, 2017 |
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Researchers
| Lixin Li | Li, Lixin | Researcher | | |
| Ahmed Atef | Atef, Ahmed | Researcher | | |
| Agnieszka Piatek | Piatek, Agnieszka | Researcher | | |
| Zahir Ali | Ali, Zahir | Researcher | | |
| Marek Piatek | Piatek, Marek | Researcher | | |
| Mustapha Aouida | Aouida, Mustapha | Researcher | | |
| Altanbadralt Sharakuu | Sharakuu, Altanbadralt | Researcher | | |
| Ali Mahjoub | Mahjoub, Ali | Researcher | | |
| Guangchao Wang | Wang, Guangchao | Researcher | | |
| Suhail Khan | Khan, Suhail | Researcher | | |
| Nina Fedoroff | Fedoroff, Nina | Researcher | | |
| Jian-Kang Zhu | Zhu, Jian-Kang | Researcher | | |
| Magdy Mahfouz | Mahfouz, Magdy | Researcher | | |
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