Document Details

Document Type : Article In Journal 
Document Title :
Cloning and Characterization of a Critical Regulator for Preharvest Sprouting in Wheat
Cloning and Characterization of a Critical Regulator for Preharvest Sprouting in Wheat
 
Document Language : English 
Abstract : Sprouting of grains in mature spikes before harvest is a major problem in wheat (Triticum aestivum) production worldwide. We cloned and characterized a gene underlying a wheat quantitative trait locus (QTL) on the short arm of chromosome 3A for preharvest sprouting (PHS) resistance in white wheat using comparative mapping and map-based cloning. This gene, designated TaPHS1, is a wheat homolog of a MOTHER OF FLOWERING TIME (TaMFT)-like gene. RNA interference-mediated knockdown of the gene confirmed that TaPHS1 positively regulates PHS resistance. We discovered two causal mutations in TaPHS1 that jointly altered PHS resistance in wheat. One GT-to-AT mutation generates a mis-splicing site, and the other A-to-T mutation creates a premature stop codon that results in a truncated nonfunctional transcript. Association analysis of a set of wheat cultivars validated the role of the two mutations on PHS resistance. The molecular characterization of TaPHS1 is significant for expediting breeding for PHS resistance to protect grain yield and quality in wheat production. 
ISSN : 1943-2631 
Journal Name : GENETICS 
Volume : 195 
Issue Number : 1 
Publishing Year : 1434 AH
2013 AD 
Article Type : Article 
Added Date : Tuesday, July 25, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Shubing LiuLiu, Shubing Researcher  
Sunish SehgalSehgal, Sunish Researcher  
Jiarui LiLi, Jiarui Researcher  
Meng LinLin, Meng Researcher  
Harold TrickTrick, Harold Researcher  
Jianming YuYu, Jianming Researcher  
Bikram GillGill, Bikram Researcher  
Guihua BaiBai, Guihua Researcher  

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