Document Details

Document Type : Article In Journal 
Document Title :
Importance of the methyl-citrate cycle on glycerol metabolism in the yeast Yarrowia lipolytica
Importance of the methyl-citrate cycle on glycerol metabolism in the yeast Yarrowia lipolytica
 
Document Language : English 
Abstract : A novel approach to trigger lipid accumulation and/or citrate production in vivo through the inactivation of the 2-methyl-citrate dehydratase in Yarrowia lipolytica was developed. In nitrogen-limited cultures with biodiesel-derived glycerol utilized as substrate, the Delta phd1 mutant (JMY1203) produced 57.7 g/L of total citrate, 1.6-fold more than the wild-type strain, with a concomitant glycerol to citrate yield of 0.91 g/g. Storage lipid in cells increased at the early growth stages, suggesting that inactivation of the 2-methyl-citrate dehydratase would mimic nitrogen limitation. Thus, a trial of JMY1203 strain was performed with glycerol under nitrogen-excess conditions. Compared with the equivalent nitrogen-limited culture, significant quantities of lipid (up to similar to 31% w/w in dry weight, 1.6-fold higher than the nitrogen-limited experiment) were produced. Also, non-negligible quantities of citric acid (up to similar to 26 g/L, though 0.57-fold lower than the nitrogen-limited experiment) were produced, despite remarkable nitrogen presence into the medium, indicating the construction of phenotype that constitutively accumulated lipid and secreted citrate in Y. lipolytica during growth on waste glycerol utilized as substrate. 
ISSN : 0168-1656 
Journal Name : JOURNAL OF BIOTECHNOLOGY 
Volume : 168 
Issue Number : 4 
Publishing Year : 1434 AH
2013 AD 
Article Type : Article 
Added Date : Sunday, July 23, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Seraphim PapanikolaouPapanikolaou, Seraphim Researcher  
Athanasios BeopoulosBeopoulos, Athanasios Researcher  
Anna KolettiKoletti, Anna Researcher  
France ThevenieauThevenieau, France Researcher  
Apostolis KoutinasKoutinas, Apostolis Researcher  
Jean-Marc NicaudNicaud, Jean-Marc Researcher  
George AggelisAggelis, George Researcher  

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