Document Details

Document Type : Article In Journal 
Document Title :
Characterization of a polygalacturonase from trichoderma harzianum grown on citrus peel with application for apple juice
Characterization of a polygalacturonase from trichoderma harzianum grown on citrus peel with application for apple juice
 
Subject : Biochemistry 
Document Language : English 
Abstract : A polygalacturonase (PGase) was purified from Trichoderma harzianum, grown on citrus peel, by anion exchange and size exclusion chromatographies. The enzyme had molecular mass of 29 kDa. The extent of hydrolysis by PGase was decreased with increasing of pectin esterification degree. Km and Vmax, using polygalacturonic acid as substrate, were 1.42 mg and 0.66 μmol reducing sugar, respectively. PGase had a broad pH optimum ranged from 4.5 to 7.0 and temperature optimum at 55°C. PGase was stable up to 50°C after incubation for 30 min. All the examined metal cations showed partial inhibitory effects, except for Hg2+which was completely inhibited the enzyme activity. The effect of PGase and wheat α-amylase on apple juice was studied, where the mixture of the two enzymes increased the quantity (135% fold) and clarity degree of apple juice. 
ISSN : 1991-8178 
Journal Name : Australian Journal of Basic and Applied Sciences 
Volume : 3 
Issue Number : 3 
Publishing Year : 1430 AH
2009 AD 
Article Type : Article 
Added Date : Sunday, December 25, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
صالح أحمد محمدMohamed, Saleh AhmedResearcherDoctoratesaleh38@hotmail.com
عبدالرحمن لبيد المالكيAl-Malki, Abdulrahman LResearcherDoctoratealalmalki@kau.edu.sa
طه عبدالله قمصانيKumosani, Taha AbdullahResearcherDoctoratetkumosani@kau.edu.sa

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