Document Details

Document Type : Article In Journal 
Document Title :
Inhibition of lipid peroxidation in UVA-treated skin fibroblasts by luteolin and its glucosides
Inhibition of lipid peroxidation in UVA-treated skin fibroblasts by luteolin and its glucosides
 
Subject : Biochemistry 
Document Language : English 
Abstract : Ultraviolet A (UVA) radiation causes oxidative damage to human skin cells. This damage may be reduced or prevented using plant compounds as photoprotectants. To investigate the relationship between chemical structure and UVA-protective activity, three structurally related flavonoids, namely luteolin, luteolin-7-O-glucoside (both present in artichoke) and luteolin-4′-O-glucoside (present in wild carrot), were studied. Human skin fibroblasts exposed to UVA (250 and 500 kJ/m2) were treated with each flavonoid (30 μM) for 18 h prior to irradiation. The extent of lipid peroxidation in the cellular extracts was assessed as lipid peroxides and malondialdehyde (MDA). Luteolin and luteolin-7-O-glucoside both prevented a significant increase in lipid peroxides at 250 kJ/m2, but at 500 kJ/m2 their effectiveness was clearly attenuated. Contrastingly, luteolin-4′-O-glucoside was pro-oxidant at both radiation doses. Measurements of MDA levels highlighted that luteolin was clearly more effective than the two glucosides at both 250 and 500 kJ/m2. Overall, these results show clear differences between the three flavonoids and suggest that the B ring 3′,4′-dihydroxy group, lacking in luteolin-4′-O- glucoside, may be particularly important. Flavonoid: transition metal ion chelation studies confirmed the influence of the 3′,4′-dihydroxy group, which is also relevant to the quenching of singlet oxygen. These features as well as the greater lipophilic nature of luteolin together explain the superior activity of this flavonoid which may be potentially useful as a supplement in photoprotective skin preparations. 
ISSN : 1438-7697 
Journal Name : European Journal of Lipid Science and Technology 
Volume : 109 
Issue Number : 7 
Publishing Year : 1428 AH
2007 AD 
Article Type : Article 
Added Date : Wednesday, December 21, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
سوسن عبدالعزيز رحيم الدينRahimuddin, Sawsan AResearcherDoctoratesrahimaldeen@kau.edu.sa
سمير محمد خوجهKhoja, Samir MResearcherDoctorateskhoja@kau.edu.sa
زهير محمد المرزوقيMarzoki, Zuhair MResearcherDoctorate 
Nazlin K HowellHowell, Nazlin KResearcherDoctorate 
Jonathan E BrownBrown, Jonathan EResearcherDoctoratej.e.brown@surrey.ac.uk

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