Document Details

Document Type : Article In Journal 
Document Title :
Synthesis of a new β-naphthothiazole monomethine cyanine dye for the detection of DNA in aqueous solution
Synthesis of a new β-naphthothiazole monomethine cyanine dye for the detection of DNA in aqueous solution
 
Subject : Chemistry 
Document Language : English 
Abstract : Novel monomethine cyanine dye (MC) derived from β-naphthothiazole and benzothiazole has been prepared and characterized by 1H and 13C NMR, FTIR, ESIMS, elemental analyses, absorption and fluorescence spectroscopy. The dye was conveniently synthesized by the condensation of two sulfate heterocyclic quaternary salts. The interaction between calf thymus DNA (ct-DNA) in tris(hydroxymethyl)aminomethane-HCl (Tris-HCl) aqueous buffer solution and MC has been studied with spectral fluorescence method. The binding constant value has been determined by fluorescence titration of MC with ct-DNA concentrations. The result obtained is consistent with an intercalative binding interaction between MC and ct-DNA. Compared with ethidium bromide (EB), MC showed a huge fluorescence enhancement upon mixing with ct-DNA. 
ISSN : 1386-1425 
Journal Name : Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy 
Volume : 75 
Issue Number : 5 
Publishing Year : 1431 AH
2010 AD 
Article Type : Article 
Added Date : Tuesday, October 4, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
رضا محمد الششتايEl-Shishtawy, Reda MohamedResearcherDoctorateelshishtawy@hotmail.com
عبدالله محمد عسيريAsiri, Abdullah MohamedResearcherDoctorateaasiri2@kau.edu.sa
سالم أحمد باسيفBasief, Salem AResearcherDoctoratesbasaif@yahoo.com
طارق رشاد سبحيSobahi, Tariq RashadResearcherDoctoratedrtariq_s@hotmail.com

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