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Document Type |
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Thesis |
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Document Title |
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moecular genetic study for early of human breast cancer دراسة وراثية جزيئية للتشخيص المبكر لسرطان الثدي البشري. |
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Subject |
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Biological |
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Document Language |
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Arabic |
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Abstract |
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Breast cancer is the most frequent cancer in women world wide. A growing interest in the use of molecular markers in order to assess prognosis of breast cancer is taking place. According to most recent studies, there is a positive relationship between increased frequency of mutations in BRACA1 and BRACA2 genes and the causes of breast cancer. The current work aims to correlate any nucleotide change observed within exons (10 & 11) in BRCA1 and BRCA2 genes (that represent most areas of mutations in the two genes) with causative etiological factors ,for a chosen group of breast cancer patients respectively, from the Western region of Saudi Arabia. And immunohistochemistry analysis was performed as a confirmatory test for the over expression of HER-2 neu and BRCA1 proteins. The DNA was extracted from the 60 paraffin-embedded tissues and the 20 blood samples of sporadic and familial (family history) breast invasive ductal carcinoma (BIDC). The sporadic breast cancer patient's age ranged between 30-72 years. And the familial breast cancer patients aged between 30-62 years. Polymerase chain reaction (PCR) was performed. Single stranded conformation polymorphisms (SSCPs) assay showed band shift for 18/60 (30%) of the sporadic tumor samples, but mutations detected by DNA sequencing in 11nucleotides only, representing 9/60 (15%). There were 4/11 (36.36 %) in BRCA1 exon 11, 4/11 (36.36) in BRCA2 exon 10 and 3/11(27.28 %) in BRCA2 exon 11. While in the 20 familial breast cancer patients, band shifts were identified in 10/20 (50%), but mutations detected in 7 nucleotide in 7/20 (35%) tumor samples, 7 mutations, 4 in BRCA1 exon 11 (57.1%), 2 in BRCA2 exon 10 (28.6 %), 1 in BRCA2 exon 11(14.3%). No mutations were detected in BRCA1 and exon 10 for the sporadic and the familial breast cancer. It was demonstrated that in sporadic breast cancer patients, immunohistochemistry of breast cancer patients for HER-2/neu expression was much better than BRCA1 expression, for patient follow-up, in the absence of high and sensitive technologies. The conclution of this study showed that ,all females from age 30 and more should watch breast cancer by doing monthly self-breast examination because the type of breast cancer discovered in the KSA is more aggressive from other countries. |
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Supervisor |
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Prof. nada hassan altwaty alqourashy |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1430 AH
2009 AD |
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Added Date |
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Sunday, December 5, 2010 |
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Researchers
| ديانا علي القويعي | ALQUWAIE, DIANA ALI | Researcher | Master | |
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