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Document Type |
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Thesis |
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Document Title |
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Genetic variantions at the visfatin locus and risk of colon cancer in Saudi population التغيرات الوراثية لموقع جين الفسفاتين وخطورة سرطان القولون لدى المجتمع السعودي |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Visfatin, an adipocytokine predominantly secreted from visceral adipocytes , has a strong genetic component. In this study we thought to evaluate the association between two single nucleotide polymorphisms (SNPs), rs9770242 and rs4730153, in the visfatin gene with the risk of colon cancer in the Saudi population. The study is also designed to assess whether the two SNPs contribute to circulating visfatin or the level of visfatin is independently associated with colon cancer risk. To the best of our knowledge this is the first report on visfatin gene and colon cancer risk. Colon cancer patients (n=80 ; age 55.71±12.87 yr; BMI 25.73±5.73 kg/m2) and control subjects (n=70; age 50.6±11.74 yr; BMI 28.58±6.25 kg/m2) were genotyped using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) techniques. In comparison with the control group, the colon cancer group displayed a different genotype distribution of the visfatin rs9770242 and rs4730153. Carriers of the heterozygous (AC) genotype of rs9770242 had almost fourfold (OR=3.9, 95% CI 1.5 – 4.6, p=0.3) and the homozygous (CC) genotype had more than a fivefold (OR=5.7, 95% CI 1.10 – 22.14, p=0.23) higher colon cancer risk than carriers of the normal (AA) genotype. Also, SNP rs4730153 heterozygous (AG) genotype had more than a fourfold (OR= 4.68, 95% CI 1.56 – 4.35, p=0.36) and the homozygous (GG) genotype had more than a fivefold (OR=5.82, 95% CI 1.61 – 10.25, p=0.33) higher colon cancer risk than carriers of the normal (AA) genotype. This study showed that serum visfatin levels were significantly (P=0.0001) higher in the colon cancer patients than in the control group, and this concentration is higher (P=0.04) in females than males in control group only. Interestingly enough, in our study the increase in the visfatin concentrations has an association with the rs9770242 and rs4730153 genotyping. In conclusion, our finding suggests a potential role for visfatin rs4730153 and rs9770242 SNPs in the genetic predisposition to colon cancer disease among Saudi population. In addition, serum visfatin levels were significantly associated with colon cancer risk and with the rs9770242 and rs4730153 genotyping. Our results propose that visfatin may be one of the clinically important proteins associated with colon cancer disease. |
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Supervisor |
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Dr. Rowyda Nawwaf Al-Harithy |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1431 AH
2010 AD |
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Added Date |
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Saturday, December 4, 2010 |
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Researchers
| رنا عبدالله الغامدي | Al-Ghamdi, Rana Abdullah | Researcher | Master | |
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