Document Details

Document Type : Article In Journal 
Document Title :
Cytogenetics and multiple chromosomes painting studies of human lymphoma cell lines.
Cytogenetics and multiple chromosomes painting studies of human lymphoma cell lines.
 
Document Language : English 
Abstract : The use of cell lines can be of considerable importance in studying the genetics of cancer. However, the detection of chromosome abnormalities in malignant cell lines by conventional cytogenetics is particularly problematic because of the difficulty in routinely preparing metaphase spreads of sufficient quality or quantity and because of the complex nature of many of the chromosomal changes. Fluorescence in-situ hybridization (FISH) is a powerful tool for improving the analysis of malignant cell chromosomes (Hammond et al., Ann. Oncol., 1994, 5 suppl.l, 51-54) and we have therefore adopted this approach in our studies of lymphoma cells. In this study, we have analysed five human lymphoma cell lines (Daudi, HS 602, Raji, MC1 16 and Namalwa). Some of these were partially analysed cytogenetically in the early 1970s using conventional staining techniques, but no chromosome analysis had been previously attempted on the Raji and HS 602 cell lines. Initially we completed the cytogenetic analysis of each of these cell lines using the G-banding technique. We then refined these preliminary findings by applying multicolour FISH analysis with whole chromosome paint probes for all twenty three human chromosome types using direct and indirect combinatorial labelling with two fluorochromes (FITC and Texas Red). In this way we were able to simultaneously visualise multiple target chromosomes in the same cell. These studies are still in progress, but they have already enabled complex rearrangements such as an ins(lp), ins(3p) and dup(lp)to be characterised and made the identification of marker chromosomes e.g. as a t(6q;7q) possible. 
ISSN : 1532-1827 
Journal Name : British Journal of Cancer 
Volume : 75 
Issue Number : 1 
Publishing Year : 1418 AH
1997 AD 
Article Type : Article 
Added Date : Monday, May 3, 2010 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
محمد حسين القحطانيAl-Qahtani, Mohammed HussainInvestigatorDoctoratemhalqahtani@kau.edu.sa

Back To Researches Page